Using a kit developed for high school students, NYC DIY BIO transform E. coli with a plasmid carrying the gene for Green Fluorescent Protein (GFP). It's a little hard to understand at times, but the on screen titles help out. Great work NYC DIY BIO!
The work area met all criteria for Biosafety Level 1 as defined by the Centers for Disease Control.
In the last video they finish the transformation of E. coli with a plasmid containing the GFP (Green Fluorescent Protein) gene and succeed in generating green bacterial colonies. Next meeting we will purify the GFP and visualize it under black light and with gel electrophoresis.
For more info on GFP check out these archived posts:
1. BIO LAB EXPERIMENT WALK THROUGH VIDEOS - Mike White's Entire Collection - Inserting GFP into bacteria, Mixing Agar, and More!
2. Futures in Biotech 37: Just A Touch Of Green - Published on Dec 29, 2008 GREEN FLUORESCENT PROTEIN DISCOVERY and HOW ORGANISMS SENSE TOUCH
In these podcast's Marty Chalfie, the discoverer of GFP! Describes how he developed one of the most important tools of modern molecular biology, one that allows us to see inside a living cells, down to the protein level. With green fluorescent protein, or GFP, we can now track the life of a protein, from when the gene that makes the protein is turned on, to where it goes, to where it dies.
3. 11 Excellent Videos introducing GFP:
4. Green fluorescent protein Virtual Lab! Narrated in a flash classroom and Student Protocols are Included!