Sunday, June 21, 2009

Jen from Standford University shows us how to do DNA extraction from Strawberries

An excellent first time project, that is completely safe, requires only a kitchen, and will give you visible DNA in a glass. And Jennifer is delightful! The video's are so easy to follow, they work for any level and any age group. Thanks Jen, This is great.

This article is from the Home Activities section of Stanford University's understanding genetics website:
For your convenience it has been pasted below:

Do-It-Yourself Strawberry DNA (Kiwi's and Banana's work too)

Strawberries, bacteria, humans—all living things have genes, and all of these genes are made of DNA. That's why scientists can take a gene from one living thing and put it into another. For example, they can put human genes into bacteria to make new medicines.

How do scientists take DNA out of a living thing? It's not that hard—there are lots of ways to do it! You can follow the directions in the video above to get DNA out of a strawberry. Or you can follow the steps below. Either way you'll have strawberry DNA at the end!

What you need:

* measuring cup
* measuring spoons
* rubbing alcohol
* 1/2 teaspoon salt
* 1/3 cup water
* 1 tablespoon Dawn dishwashing detergent
* glass or small bowl
* cheesecloth
* funnel
* tall drinking glass
* 3 strawberries (green tops removed)
* reclosable plastic sandwich bags
* test tube or small glass jar (like the kind spices come in)
* bamboo skewer (find them at the grocery store)

What to do:

1. Chill the rubbing alcohol in the freezer. (You'll need it later.)
2. Mix the salt, water, and Dawn detergent in a glass or small bowl. Set the mixture aside. This is your extraction liquid.
3. Line the funnel with the cheesecloth, and put the funnel's tube into the glass.
4. Put the strawberries in the plastic bag and push out all the extra air. Seal it tightly.
5. With your fingers, squeeze and smash the strawberry mixture for 2 minutes.
6. Add 3 tablespoons of the extraction liquid you made in Step 2 to the strawberries in the bag. Push out all the extra air and reseal the bag.
7. Squeeze the strawberry mixture with your fingers for 1 minute.
8. Pour the strawberry mixture from the bag into the funnel. Let it drip into the glass until there is no liquid left in the funnel.
9. Throw away the cheesecloth and the strawberry pulp inside. Pour the contents of the glass into the test tube or small glass jar so it is 1/4 full.
10. Tilt the test tube or jar and very slowly pour the cold rubbing alcohol down the side. The alcohol should form a layer on top of the strawberry liquid. (Don't let the alcohol and strawberry liquid mix. The DNA collects between the two layers!)
11. Dip the bamboo skewer into the test tube where the alcohol and strawberry layers meet. Pull up the skewer. The whitish, stringy stuff is DNA containing strawberry genes!

You can try these steps to purify DNA from lots of other living things. Grab some oatmeal or kiwis from the kitchen and try it again! Which foods give you the most DNA?

And here's the DIY BIO NYC Group doing Strawberry DNA extraction in shot and wine glasses, and trying out a homemade gel electrophoresis box.

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